LOCUS       sequence-7976-ex        9122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 9122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GAL-EGFP-ccdB
FEATURES             Location/Qualifiers
     source          1..9122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2755..2775
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2757..2775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2799..3240
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2799..2916
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3211..3234
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     3255..3271
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3255..3271
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(3284..3286,3287..3289,3290..4000)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3284..3286,3287..3289,3290..4000)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3329..3350)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3590..3609)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3937..3958
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4025..4149
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4174..4179,4180..4197,4198..4204)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4258..4917
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4324..4343)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5262..5567
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5446..5465
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5608..5732)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5739..5986
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5755..5773)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6004..6023)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6005..6023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6033..6050)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6033..6049)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6042..6064)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6194..6649
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6281..6300)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6491..6512
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6949..7353
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7366..8460
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(8953..8972)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9072..9094
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag ctctagtacg gattagaagc cgccgagcgg
     2821 gcgacagccc tccgacggaa gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc
     2881 ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta
     2941 gcttttatgg ttatgaagag gaaaaattgg cagtaacctg gccccacaaa ccttcaaatt
     3001 aacgaatcaa attaacaacc ataggatgat aatgcgatta gttttttagc cttatttctg
     3061 gggtaattaa tcagcgaagc gatgattttt gatctattaa cagatatata aatggaaaag
     3121 ctgcataacc actttaacta atactttcaa cattttcagt ttgtattact tcttattcaa
     3181 atgtcataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa cgtcaaggag
     3241 aaaaaacccc ggattctaga actagtggat cccccgggac aaaatggtga gcaagggcga
     3301 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3361 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3421 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3481 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3541 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3601 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3661 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3721 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3781 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3841 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3901 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3961 cgccgccggg atcactctcg gcatggacga gctgtacaag ggtggaccag gtggtggaca
     4021 tcaaacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     4081 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     4141 gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     4201 aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg
     4261 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     4321 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     4381 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     4441 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
     4501 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     4561 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     4621 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     4681 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     4741 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     4801 aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg cttccatgtc
     4861 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg
     4921 ccgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
     4981 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta
     5041 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
     5101 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
     5161 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
     5221 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca
     5281 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     5341 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
     5401 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     5461 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     5521 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
     5581 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat
     5641 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
     5701 gtttctcgtt cagctttctt gtacaaagtg gttcgatgtc atgtaattag ttatgtcacg
     5761 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
     5821 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
     5881 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
     5941 aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcggcc ggtacccaat
     6001 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     6061 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6121 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     6181 ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6241 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6301 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6361 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6421 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6481 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6541 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6601 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ctgatgcggt
     6661 attttctcct tacgcatctg tgcggtattt cacaccgcat atcgacggtc gaggagaact
     6721 tctagtatat ccacatacct aatattattg ccttattaaa aatggaatcc caacaattac
     6781 atcaaaatcc acattctctt caaaatcaat tgtcctgtac ttccttgttc atgtgtgttc
     6841 aaaaacgtta tatttatagg ataattatac tctatttctc aacaagtaat tggttgtttg
     6901 gccgagcggt ctaaggcgcc tgattcaaga aatatcttga ccgcagttaa ctgtgggaat
     6961 actcaggtat cgtaagatgc aagagttcga atctcttagc aaccattatt tttttcctca
     7021 acataacgag aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt
     7081 tttgttaatt tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa
     7141 aaaggaaagg tgagaggccg gaaccggctt ttcatataga atagagaagc gttcatgact
     7201 aaatgcttgc atcacaatac ttgaagttga caatattatt taaggaccta ttgttttttc
     7261 caataggtgg ttagcaatcg tcttactttc taacttttct taccttttac atttcagcaa
     7321 tatatatata tatttcaagg atataccatt ctaatgtctg cccctatgtc tgcccctaag
     7381 aagatcgtcg ttttgccagg tgaccacgtt ggtcaagaaa tcacagccga agccattaag
     7441 gttcttaaag ctatttctga tgttcgttcc aatgtcaagt tcgatttcga aaatcattta
     7501 attggtggtg ctgctatcga tgctacaggt gtcccacttc cagatgaggc gctggaagcc
     7561 tccaagaagg ttgatgccgt tttgttaggt gctgtgggtg gtcctaaatg gggtaccggt
     7621 agtgttagac ctgaacaagg tttactaaaa atccgtaaag aacttcaatt gtacgccaac
     7681 ttaagaccat gtaactttgc atccgactct cttttagact tatctccaat caagccacaa
     7741 tttgctaaag gtactgactt cgttgttgtc agagaattag tgggaggtat ttactttggt
     7801 aagagaaagg aagacgatgg tgatggtgtc gcttgggata gtgaacaata caccgttcca
     7861 gaagtgcaaa gaatcacaag aatggccgct ttcatggccc tacaacatga gccaccattg
     7921 cctatttggt ccttggataa agctaatgtt ttggcctctt caagattatg gagaaaaact
     7981 gtggaggaaa ccatcaagaa cgaattccct acattgaagg ttcaacatca attgattgat
     8041 tctgccgcca tgatcctagt taagaaccca acccacctaa atggtattat aatcaccagc
     8101 aacatgtttg gtgatatcat ctccgatgaa gcctccgtta tcccaggttc cttgggtttg
     8161 ttgccatctg cgtccttggc ctctttgcca gacaagaaca ccgcatttgg tttgtacgaa
     8221 ccatgccacg gttctgctcc agatttgcca aagaataagg ttgaccctat cgccactatc
     8281 ttgtctgctg caatgatgtt gaaattgtca ttgaacttgc ctgaagaagg taaggccatt
     8341 gaagatgcag ttaaaaaggt tttggatgca ggtatcagaa ctggtgattt aggtggttcc
     8401 aacagtacca ccgaagtcgg tgatgctgtc gccgaagaag ttaagaaaat ccttgcttaa
     8461 aaagattctc tttttttatg atatttgtac ataaacttta taaatgaaat tcataataga
     8521 aacgacacga aattacaaaa tggaatatgt tcatagggta gacgaaacta tatacgcaat
     8581 ctacatacat ttatcaagaa ggagaaaaag gaggatagta aaggaataca ggtaagcaaa
     8641 ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat taatattgac
     8701 aaggaggagg gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa ctacgattcc
     8761 taatttgata ttggaggatt ttctctaaaa aaaaaaaaat acaacaaata aaaaacactc
     8821 aatgacctga ccatttgatg gagtttaagt caataccttc ttgaaccatt tcccataatg
     8881 gtgaaagttc cctcaagaat tttactctgt cagaaacggc cttacgacgt agtcgatatg
     8941 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     9001 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     9061 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     9121 ga
//
