LOCUS       sequence-79762-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivector that encodes H2B-eYFP under the transcriptional control 
            of the mouse TIE1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Sato Y, Poynter G, Huss D, Filla MB, Czirok A, Rongish BJ, Little 
            CD, Fraser SE, Lansford R
  TITLE     Dynamic analysis of vascular morphogenesis using transgenic quail 
            embryos.
  JOURNAL   PLoS One. 2010 Sep 14;5(9):e12674. doi: 
            10.1371/journal.pone.0012674.
  PUBMED    20856866
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-09-14"; volume: "5(9):e12674. doi"; pages: " 
            10.1371/journal.pone.0012674"
COMMENT     Sequence Label: pLenti.tie1.H2B.eYFP.W
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(38..751,752..754,755..757))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     432..451
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      754..763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgacgc ggccgcttta cttgtacagc tcgtccatgc
       61 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      121 tggggtcttt gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg
      181 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
      241 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
      301 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
      361 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
      421 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
      481 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg
      541 cgaagcactg caggccgtag ccgaaggtgg tcacgagggt gggccagggc acgggcagct
      601 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      661 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      721 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt acccggggat
      781 ccttagcgct ggtgtacttg gtgatggcct tagtaccctc ggacacggcg tgcttggcca
      841 actccccagg cagcagcagg cgcacggccg tctggatctc cctggaggtg atggtcgagc
      901 gcttgttgta atgc
//
