LOCUS       sequence-79770-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of full-length mouse Arp11 under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Eckley DM, Schroer TA
  TITLE     Interactions between the evolutionarily conserved, actin-related 
            protein, Arp11, actin, and Arp1.
  JOURNAL   Mol Biol Cell. 2003 Jul;14(7):2645-54. Epub 2003 Mar 20.
  PUBMED    12857853
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2003-07"; volume: "14(7)"; pages: "2645-54. Epub 2003
            Mar 20"
COMMENT     Sequence Label: T7-His-Arp11(1–417)
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..98
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(102..110,111..125)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc
       61 atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg
      121 ataaggatcg atccatgcat catcgtcatc atcatatgcc gctctacgag ggccttggga
      181 gcggcggcga gaagacagca gtcgtgatcg acctaggaga agctttcacc aagtgtggat
      241 tcgcaggaga aactggtcca cgatgtataa ttcctagtgt gataaaaaga gctggcatgt
      301 ctaagccaat caaagttgtt cagtataata tcaatacaga agaattatat tcctacctaa
      361 aggaattcat ccacatactg tatttcaggc atctgttggt gaatcccaga gaccgccgcg
      421 ttgtggttat cgagtcggtg ttatgtcctt cccacttcag agagactctg actcgtgttc
      481 tttttaaata ttttgaggtt ccatctgtcc tacttgctcc aagtcatctg atggcactgc
      541 tgacgcttgg aattaattcg gccatggtcc tggattgtgg atatagggaa agcctggtgt
      601 tgcctatcta tgaaggcatc ccaatactga attgctgggg agcactgccg ttaggaggaa
      661 aagctctcca caaggagttg gaaactcagc tgttggaaca atgtactgtt gacactggtg
      721 cagctaaagg acagagcctt ccctcagtga tgggttcagt tccagaaggt gtgctagaag
      781 atattaaagt gcgcacgtgc tttgtcagtg atctgaagcg tggactgcaa atccaagcag
      841 caaaatttaa tattgatggg aataatgagc gtcccactcc acctccaaat gttgactatc
      901 c
//
