LOCUS       sequence-79772-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivector that expresses H2B-chFP from internal human PGK 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Huss D, Benazeraf B, Wallingford A, Filla M, Yang J, Fraser SE, 
            Lansford R
  TITLE     Transgenic quail to dynamically image amniote embryogenesis.
  JOURNAL   Development. 2015 Jul 24. pii: dev.121392.
  PUBMED    26209648
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2015 Jul 24. pii: dev.121392."
COMMENT     Sequence Label: pLenti.PGK.H2B-chFP.W
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..452
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      465..474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(616..634)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cgaatcaccg acctctctcc ccagggggat ctcgagctca agcttcgaat tctgcagtcg
       61 acggtaccgc caccatgcca gagccagcga agtctgctcc cgccccgaaa aagggctcca
      121 agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc agccgcaagg
      181 agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac accggcattt
      241 cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag cgcatcgcag
      301 gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc agggagatcc
      361 agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg tccgagggta
      421 ctaaggccat caccaagtac accagcgcta aggatccacc ggtcgccacc atggtgagca
      481 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      541 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      601 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      661 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      721 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      781 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      841 tctacaaggt gaagctgcgc ggcaccaact tcc
//
