LOCUS       sequence-79780-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of RFP-tagged amino acid 369-701 (headless) of KIF3A in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Brown CL, Maier KC, Stauber T, Ginkel LM, Wordeman L, Vernos I, 
            Schroer TA
  TITLE     Kinesin-2 is a motor for late endosomes and lysosomes.
  JOURNAL   Traffic. 2005 Dec;6(12):1114-24.
  PUBMED    16262723
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic."; 
            date: "2005-12"; volume: "6(12)"; pages: "1114-24"
COMMENT     Sequence Label: RFP-KIF3A-HL
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             53..727
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             53..727
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(183..201)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     451..470
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     645..668
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggcctc
       61 ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa
      121 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      181 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      241 tcagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
      301 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      361 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
      421 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
      481 ggaggcctcc accgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagat
      541 gaggctgaag ctgaaggacg gcggccacta cgacgccgag gtcaagacca cctacatggc
      601 caagaagccc gtgcagctgc ccggcgccta caagaccgac atcaagctgg acatcacctc
      661 ccacaacgag gactacacca tcgtggaaca gtacgagcgc gccgagggcc gccactccac
      721 cggcgcctcc ggactcagat ctcgaggaga gctgaagaaa aagcttgagg aaggggaaga
      781 agtgtcaggc tctgacatca gtgggtcaga ggaggatgac gaggagggtg aacttggaga
      841 agatggagaa aaaaagaaaa agagaaggga ccaagcaggt aaaaagaaag tttccccaga
      901 taagatggtg gaaatgcaag cgaaaattga cgaggagaga aaagcactgg aaaccaaact
      961 tgacatggaa gaagaaga
//
