LOCUS       sequence-79790-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivector that expresses H2B-cerFP from internal CAG 
            enhancer-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  TITLE     Lentivirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti.CAG.H2B-cerFP.W.SINloxP
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             146..523
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      536..545
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(587..608)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
       61 tcatcatttt ggcaaagaat tgatttgata ccgcgggccc gggatccctc gaggaattat
      121 cgaattcgag ctcggtaccg ccaccatgcc agagccagcg aagtctgctc ccgccccgaa
      181 aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg
      241 cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga
      301 caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga
      361 gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc
      421 cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt
      481 gtccgagggt actaaggcca tcaccaagta caccagcgct aaggatccac cggtcgccac
      541 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      601 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      661 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      721 cctcgtgacc accctgacct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
      781 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      841 cttcaaggac gacggcaact acaaga
//
