LOCUS       sequence-79800-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivector that expresses H2B-cerFP-2A-mito-dsRFP from internal CAG
            enhancer-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  TITLE     Lentivirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti.CAG.H2B-cerFP-2A-mito-dsRFP.W
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(32..706,707..709,710..712))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(94..117)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(292..311)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     509..529
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     561..579
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      709..718
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgaat cctacaggaa caggtggtgg cggccctcgg
       61 tgcgctcgta ctgctccacg atggtgtagt cctcgttgtg ggaggtgatg tccagcttgg
      121 agtccacgta gtagtagccg ggcagctgca cgggcttctt ggccatgtag atggacttga
      181 actccaccag gtagtggccg ccgtccttca gcttcagggc cttgtggatc tcgcccttca
      241 gcacgccgtc gcgggggtac aggcgctcgg tggaggcctc ccagcccatg gtcttcttct
      301 gcattacggg gccgtcggag gggaagttca cgccgatgaa cttcaccttg tagatgaagc
      361 agccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca
      421 tcacgcgctc ccacttgaag ccctcgggga aggacagctt cttgtagtcg gggatgtcgg
      481 cggggtgctt cacgtacacc ttggagccgt actggaactg gggggacagg atgtcccagg
      541 cgaagggcag ggggccgccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg
      601 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgc
      661 gcaccttgaa gcgcatgaac tccttgatga cgttcttgga ggagcgcacc atggtggcga
      721 ccggtggtct agagctacac aaagttcttg tggctggctt gaaaaatctt atagattgac
      781 gta
//
