LOCUS       sequence-79805-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses H2B-chFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  TITLE     Lentivirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pH2B-cherryFP
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..733)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(301..320)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     570..588
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      730..739
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgatctaga gtcgcggccg ctttacttgt acagctcgtc catgccgccg gtggagtggc
       61 ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt
      121 ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg
      181 tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct
      241 cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg
      301 tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt
      361 agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct
      421 cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg
      481 ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga
      541 tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc
      601 cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc
      661 cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc
      721 ccttgctcac catggtggcg accggtggat ccttagcgct ggtgtacttg gtgatggcct
      781 tagtaccctc ggacacggcg tgcttggcca actcccc
//
