LOCUS       sequence-79812-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 with mutated Ser and Thr residues in the 
            N-terminus and mutations at P676C I730C. Contains N-terminal 
            3xFLAG-YFP tag for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutati...
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agggtgacct ggagccgagg cccacggctc ctagctccgg cgcgccaggc ctcgccggag
       61 tcggcgaaac gccgagcgcc gctgcgctgg ccgcagccag ggtggaactg cccggaaccg
      121 ctgtgccctc ggtgccggag gatgctgcgc ccgcgagccg ggacgggggc ggggtccgcg
      181 atgagggacc agccgccgcc ggtgacggcc tgggcagacc cttggggcct accccgagcc
      241 agagccgttt ccaggtggac ctcgtttccg agaacgcagg gcgggctgct gctgcggcgg
      301 cggcggctgc tgcggccgcc gccgctgctg gtgccggggc gggggccaag cagactcctg
      361 ccgacgggga agccagcggc gagagcgagc cagctaaagg cagcgaggaa gccaaaggcc
      421 gcttccgcgt taacttcgta gatcctgctg ccagcaattc cgcagaagat cagctgtcag
      481 atgccgccgg ggtcggagtc gatggaccca acgtgcagtt ccagaacggc ggggacgctg
      541 tactgagcga gggccgtagc ctccattccg gtggcggcgg cggcgagggc caccaccagc
      601 actactatta tgatgctcac gctaaccagt actatctgcg cgctttcggc cac
//
