LOCUS       sequence-79813-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 with truncation of N-terminus and mutations at
            P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-termina...
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctctacaagg gtgacctgga gccgaggccc acggctccta gctccggcgc gcgcgccagg
       61 atcgaccact accggcacac agccgcgcag ctcggcgaga agctgctccg gcctagcctg
      121 gccgagctgc atgacgagct ggaaaaggaa ccttttgagg atggctttgc aaatggggaa
      181 gaaagcactc caacacgtga tgctgtggtc acgtacacgg ccgaaagtaa aggagtcgtg
      241 aagtttggct ggatcaaggg tgtactagta cgttgtatgt taaacatttg gggtgtgatg
      301 cttttcattc gtctctcatg gattgtggga caagctggaa ttggtctatc agtccttgta
      361 ataatgatgg ctactgttgt gacaactatc acaggattgt ccactagcgc catcgcaact
      421 aatggatttg taaggggagg cggcgcatat tatttaatat ctagaagtct agggcctgaa
      481 tttggtggtg ccattggtct aatcttcgcc tttgccaacg ctgttgctgt tgctatgtat
      541 gtggttggat ttgcagaaac cgtggtggag ttgctgaaag aacattccat tcttatgata
      601 gatgaaatca atgacatccg gattattgga gccattacag tcgtgattct tttaggtatc
      661 tcagtagccg gtatggagtg ggaagcaaaa gctcagattg
//
