LOCUS       sequence-79819-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of the human TPI Ile170Val allele in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Gruning NM, Du D, Keller MA, Luisi BF, Ralser M
  TITLE     Inhibition of triosephosphate isomerase by phosphoenolpyruvate in 
            the feedback-regulation of glycolysis.
  JOURNAL   Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232.
  PUBMED    24598263
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Open 
            Biol."; date: "2014-03-5"; volume: "4(3):130232. doi"; pages: " 
            10.1098/rsob.130232"
COMMENT     Sequence Label: p413GPD-hTPI Ile170Val
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..675
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     569..592
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     681..697
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     681..697
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctaaagggaa caaaagctgg agctcagttt atcattatca atactcgcca tttcaaagaa
       61 tacgtaaata attaatagta gtgattttcc taactttatt tagtcaaaaa attagccttt
      121 taattctgct gtaacccgta catgcccaaa atagggggcg ggttacacag aatatataac
      181 atcgtaggtg tctgggtgaa cagtttattc ctggcatcca ctaaatataa tggagcccgc
      241 tttttaagct ggcatccaga aaaaaaaaga atcccagcac caaaatattg ttttcttcac
      301 caaccatcag ttcataggtc cattctctta gcgcaactac agagaacagg ggcacaaaca
      361 ggcaaaaaac gggcacaacc tcaatggagt gatgcaacct gcctggagta aatgatgaca
      421 caaggcaatt gacccacgca tgtatctatc tcattttctt acaccttcta ttaccttctg
      481 ctctctctga tttggaaaaa gctgaaaaaa aaggttgaaa ccagttccct gaaattattc
      541 ccctacttga ctaataagta tataaagacg gtaggtattg attgtaattc tgtaaatcta
      601 tttcttaaac ttcttaaatt ctacttttat agttagtctt ttttttagtt ttaaaacacc
      661 agaacttagt ttcgacggat tctagaacta gtggatccat ggcgccctcc aggaagttct
      721 tcgttggggg aaactggaag atgaacgggc ggaagcagag tctgggggag ctcatcggca
      781 ctctgaacgc ggccaaggtg ccggccgaca ccga
//
