LOCUS       sequence-79822-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of the human TPI Ile170Thr allele in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Gruning NM, Du D, Keller MA, Luisi BF, Ralser M
  TITLE     Inhibition of triosephosphate isomerase by phosphoenolpyruvate in 
            the feedback-regulation of glycolysis.
  JOURNAL   Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232.
  PUBMED    24598263
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Open 
            Biol."; date: "2014-03-5"; volume: "4(3):130232. doi"; pages: " 
            10.1098/rsob.130232"
COMMENT     Sequence Label: p413GPD-hTPI Ile170Thr
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        31..674
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     568..591
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     680..696
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     680..696
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 taaagggaac aaaagctgga gctcagttta tcattatcaa tactcgccat ttcaaagaat
       61 acgtaaataa ttaatagtag tgattttcct aactttattt agtcaaaaaa ttagcctttt
      121 aattctgctg taacccgtac atgcccaaaa tagggggcgg gttacacaga atatataaca
      181 tcgtaggtgt ctgggtgaac agtttattcc tggcatccac taaatataat ggagcccgct
      241 ttttaagctg gcatccagaa aaaaaaagaa tcccagcacc aaaatattgt tttcttcacc
      301 aaccatcagt tcataggtcc attctcttag cgcaactaca gagaacaggg gcacaaacag
      361 gcaaaaaacg ggcacaacct caatggagtg atgcaacctg cctggagtaa atgatgacac
      421 aaggcaattg acccacgcat gtatctatct cattttctta caccttctat taccttctgc
      481 tctctctgat ttggaaaaag ctgaaaaaaa aggttgaaac cagttccctg aaattattcc
      541 cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct gtaaatctat
      601 ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt taaaacacca
      661 gaacttagtt tcgacggatt ctagaactag tggatccatg gcgccctcca ggaagttctt
      721 cgttggggga aactggaaga tgaacgggcg gaagcagagt ctgggggagc tcatcggcac
      781 tctgaacgcg gccaaggtac cggccgacac cgaggtggtt tgtgctcccc ctactgccta
      841 tatcgacttc gcccggcaga agctagatcc caagattgct gtggctgcgc agaactgcta
      901 caaagtgact aatggggctt ttactgggga gatcagccct g
//
