LOCUS       sequence-79825-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Shibata M, Lu T, Furuya T, Degterev A, Mizushima N, Yoshimori T, 
            MacDonald M, Yankner B, Yuan J
  TITLE     Regulation of intracellular accumulation of mutant Huntingtin by 
            Beclin 1.
  JOURNAL   J Biol Chem. 2006 May 19. 281(20):14474-85.
  PUBMED    16522639
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2006 May 19. 281(20):14474-85."
COMMENT     Sequence Label: pcDNA3-Beclin1
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             165..188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg
      121 gatccactag tccagtgtgg tggaattcgg ctttagctag catggactac aaggacgatg
      181 atgacaaaat ggaagggtct aagacgtcca acaacagcac catgcaggtg agcttcgtgt
      241 gccagcgctg cagccagccc ctgaaactgg acacgagttt caagatcctg gaccgtgtca
      301 ccatccagga actcacagct ccattactta ccacagccca ggcgaaacca ggagagaccc
      361 aggaggaaga gactaactca ggagaggagc catttattga aactcctcgc caggatggtg
      421 tctctcgcag attcatcccc ccagccagga tgatgtccac agaaagtgcc aacagcttca
      481 ctctgattgg ggaggtatct gatggcggca ccatggagaa cctcagccga agactgaagg
      541 tcactgggga cctttttgac atcatgtcgg gccagacaga tgtggatcac ccactctgtg
      601 aggaatgcac agatactctt ttagaccagc tggacactca gctcaacgtc actgaaaatg
      661 agtgtcagaa ctacaaacgc tgtttggaga tcttagagca aatgaatgag gatgacagtg
      721 aacagttaca gatggagcta aaggagctgg cactagagga ggagaggctg atccaggagc
      781 tggaagacgt ggaaaagaac cgcaagatag tggcagaaaa tctcgagaag gtccaggctg
      841 aggctgagag actggatcag gaggaagctc agtatcagag agaatacagt gaatttaaac
      901 gacagcagct ggagctggat gatgagctg
//
