LOCUS       sequence-79853-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Component of the Bxb1-rcCre binary site-specific recombinase system,
            reverse complementary Cre CDS flanked by Bxb1 attB/P recognition 
            sites in head-to-head orientation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-attBr-Cre-rc-attP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    35..84
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     CDS             complement(97..120)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgccttcttc tttttcctac agatccttaa ttaatcggcc ggcttgtcga cgacggcggt
       61 ctccgtcgtc aggatcatcc gggctgacct gacctactta tcgtcgtcat ccttgtaatc
      121 tccccatcct cgagcagcct caccatggcc ccagtctcag agtccaggtt tctgatgtag
      181 ttcatcacaa tgttcacatt ggtccagcca ccagcctgca tgatttcagg gatggacaca
      241 ccagccctgg ccatgtccct ggcagcaccc actctggcag agtggccaga ccaggccagg
      301 tatctctgcc cagagtcatc cttggcacca tagatcaggc ggtgggtggc ctcaaagatc
      361 ccttccaggg cccgggtgga cagttgggag gtggcagaag gggcagccac accattcttt
      421 ctgacccggc agaacaggta gttgttgggg tcatcagcca caccagacac agagatccat
      481 ctctccacca gcttggtaac ccccagggac agggccttct ccacaccagc tgtggacacc
      541 agggtcttgg tcctgccaat gtggatcagc attctcccac catcggtgcg ggagatgtcc
      601 ttcactctga ttctggcaat ttcggcaatg cgcagcaggg tgttgtaggc aatgcccagg
      661 aaggccaggt tcctgatgtc ctggcatctg tcagagttct ccatcaggga tctgacttgg
      721 tcaaagtcag tgcgttcaaa ggccagggcc tgcttggctc tctccccagc atccacattc
      781 tcctttctga ttctcctcat caccagggac acagcattgg agtcagaagg gcgaggcagg
//
