LOCUS       sequence-79854-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bxb1-responsive fluorescent reporter plasmid containing reverse 
            complementary ZsGreen flanked by Bxb1 attB/P recognition sites in 
            head-to-head orientation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-attBr-ZsGreen-attP
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    35..84
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     CDS             complement(92..787)
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTNNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     protein_bind    800..847
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     primer_bind     complement(867..884)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tgccttcttc tttttcctac agatccttaa ttaatcggcc ggcttgtcga cgacggcggt
       61 ctccgtcgtc aggatcatcc gggctgacgg ttcagggcaa ggcggagccg gaggcgatgg
      121 cgtgctcggt caggtgccac ttctggttct tggcgtcgct gcggtcctcg cgggtcagct
      181 tgtgctggat gaagtgccag tcgggcatct tgcggggcac ggacttggcc ttgtacacgg
      241 tgtcgaactg gcagcgcaag cggccaccgt ccttcagcag caggtacatg ctcacgtcgc
      301 ccttcaagat gccctgcttg ggcacgggga tgatcttctc gcaggagggc tcccagttgt
      361 tggtcatctt cttcatcacg gggccgtcgg cggggaagtt cacgccgtag aacttggact
      421 cgtggtacat gcagttctcc tccacgctca cggtgatgtc ggcgttgcag atgcacacgg
      481 cgccgtcctc gaacaggaag gagcggtccc aggtgtagcc ggcggggcag gagttcttga
      541 agtagtcgac gatgtcctgg gggtactcgg tgaacacgcg gttgccgtac atgaaggcgg
      601 cggacaagat gtcctcggcg aagggcaagg ggccgccctc caccacgcac aggttgatgg
      661 cctgcttgcc cttgaagggg tagccgatgc cctcgccggt gatcacgaac ttgtggccgt
      721 ccacgcagcc ctccatgcgg tacttcatgg tcatctcctt ggtcaggccg tgcttggact
      781 gggccatggt ggtggcggcg gtttgtaccg tacaccactg agaccgcggt ggttgaccag
      841 acaaaccacg agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctg
//
