LOCUS       sequence-79859-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the site-specific recombinase B3 (mouse 
            codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-NLS-HA-B3
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             73..93
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgccttcttc tttttcctac agatccttaa ttaataatac gactcactat agggggtcga
       61 cccgccacca tgcctaagaa gaagaggaag gtttacccat acgatgttcc agattacgct
      121 ggatccagca gctatatgga tctggtggat gacgagcccg caacactgta tcacaagttt
      181 gtcgaatgtc tgaaggcagg agagaatttc tgcggcgaca agctgtccgg aatcattaca
      241 atggccatcc tgaaagccat taaggctctg actgaggtga agaaaaccac cttcaacaag
      301 tacaaaacta ccatcaagca ggggctgcag tatgatgtgg gcagctccac catttctttc
      361 gtctaccacc tgaaagactg tgatgagctg agtcgcggcc tgtcagacgc ttttgaacct
      421 tacaagttca aaatcaagag taataaggag gcaaccagct tcaagacact gttcaggggc
      481 cccagcttcg gcagccagaa gaactggcga aagaaagagg tggatagaga agtggacaat
      541 ctgttccata gcaccgagac agatgaatcc atctttaagt tcattctgaa cacactggat
      601 agcatcgaga ctcagaccaa tacagaccgc cagaaaacag tgctgacttt cattctgctg
      661 atgaccttct ttaactgctg taggaacaat gatctgatga atgtggaccc cagcaccttc
      721 aagatcgtga aaaacaagtt cgtcggctac ctgctgcagg ctgaggtgaa acagactaag
      781 acccgaaaaa gcaggaacat cttctttttc cctatcagag aaaaccggtt tgatctgttc
      841 ctggcactgc acgacttttt cagaacttgc cagccta
//
