LOCUS       sequence-79867-e        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral packaging plasmid; Contains Gag and Pol; 
            also requires pRSV-Rev (Addgene#12253) and envelope expressing 
            plasmid (Addgene#12259).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L
  TITLE     A third-generation lentivirus vector with a conditional packaging 
            system.
  JOURNAL   J Virol. 1998 Nov . 72(11):8463-71.
  PUBMED    9765382
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1998 Nov . 72(11):8463-71."
COMMENT     Sequence Label: pMDLg/pRRE
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcactttggc aaagcacgtg agatctgaat tcgagatctg ccgccgccat gggtgcgaga
       61 gcgtcagtat taagcggggg agaattagat cgatgggaaa aaattcggtt aaggccaggg
      121 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
      181 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
      241 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
      301 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
      361 gaggaagagc aaaacaaaag taagaaaaaa gcacagcaag cagcagctga cacaggacac
      421 agcaatcagg tcagccaaaa ttaccctata gtgcagaaca tccaggggca aatggtacat
      481 caggccatat cacctagaac tttaaatgca tgggtaaaag tagtagaaga gaaggctttc
      541 agcccagaag tgatacccat gttttcagca ttatcagaag gagccacccc acaagattta
      601 aacaccatgc taaacacagt ggggggacat caagcagcca tgcaaatgtt aaaagagacc
      661 atcaatgagg aagctgcaga atgggataga gtgcatccag tgcatgcagg gcctattgca
      721 ccaggccaga tgagagaacc aaggggaagt gacatagcag gaactactag tacccttcag
      781 gaacaaatag gatggatgac acataatcca cctatcccag taggagaaat ctataaaaga
      841 tggataatcc tgggattaaa taaaatagta agaatgtata gccctaccag cattctggac
      901 ataagacaag gaccaaagga accctttaga gactatgtag accgattcta taaaactcta
      961 agagccgagc aagcttcaca agaggtaaaa aattggatga cagaaacctt gttggtccaa
     1021 aatgcgaacc cagattgtaa gactatttta aaagcattgg gaccaggagc gacactagaa
     1081 gaaatgatga cagcatgtca gggagtgggg ggac
//
