LOCUS       sequence-79878-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Component of the Co-Driver binary site-specific recombinase system, 
            expression of the site-specific recombinase Dre (mouse 
            codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-NLS-HA-Dre
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             79..99
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgttcatgcc ttcttctttt tcctacagat ccttaattaa taatacgact cactataggg
       61 ggtcgacccg ccaccatgcc taagaagaag aggaaggttt acccatacga tgttccagat
      121 tacgctggat cctctgagct gattattagt ggttcatctg gtggattcct gcgaaacatc
      181 ggcaaagagt atcaggaggc cgctgaaaac ttcatgaggt ttatgaatga ccagggggcg
      241 tacgctccta acactttgag ggatttgagg ttggtctttc atagctgggc cagatggtgc
      301 catgctcggc agcttgcatg gtttccaatt agtcctgaaa tggcacgcga atactttctt
      361 cagttgcacg atgcagacct ggcctccact accatcgaca agcactatgc tatgcttaat
      421 atgcttctgt cccactgcgg actgccaccc ttgtccgacg acaagtcagt gagtcttgcc
      481 atgagaagaa ttagaagaga agccgcaacc gaaaagggtg agaggacagg acaggcaatc
      541 cccctgcgct gggacgacct gaagctgctg gatgtgctgc tcagcaggag cgagcggctg
      601 gtcgacctgc gcaacagggc tttcctgttc gtagcctata acaccctcat gagaatgtct
      661 gaaatatcac gcatcagggt tggggacttg gatcagacag gagacacagt gaccctgcac
      721 atcagtcaca ctaagacaat caccacagct gcgggccttg acaaagtgct ctcccggcga
      781 accacagcag tgctcaatga ctggctggac gtcagtgggc ttagagaaca tccagacgct
      841 gtgctcttcc cacctataca ccggtc
//
