LOCUS       sequence-79881-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of the site-specific recombinase Dre and BFP under the
            control of the Thy1.2 expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: Thy1.2-NLS_HA_Dre-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             162..182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             183..209
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aagagagggg ctgaggtatt catcatgtgc tccgtggatc tcaagccctc aaggtaaatg
       61 gggacccacc tgtcctacca gctggctgac ctgtagcttt ccccaccaca gaatccaagt
      121 cggaactctt ggcacctaga ggatctcgag ccgccaccat gcctaagaag aagaggaagg
      181 tttacccata cgatgttcca gattacgctg gatcctctga gctgattatt agtggttcat
      241 ctggtggatt cctgcgaaac atcggcaaag agtatcagga ggccgctgaa aacttcatga
      301 ggtttatgaa tgaccagggg gcgtacgctc ctaacacttt gagggatttg aggttggtct
      361 ttcatagctg ggccagatgg tgccatgctc ggcagcttgc atggtttcca attagtcctg
      421 aaatggcacg cgaatacttt cttcagttgc acgatgcaga cctggcctcc actaccatcg
      481 acaagcacta tgctatgctt aatatgcttc tgtcccactg cggactgcca cccttgtccg
      541 acgacaagtc agtgagtctt gccatgagaa gaattagaag agaagccgca accgaaaagg
      601 gtgagaggac aggacaggca atccccctgc gctgggacga cctgaagctg ctggatgtgc
      661 tgctcagcag gagcgagcgg ctggtcgacc tgcgcaacag ggctttcctg ttcgtagcct
      721 ataacaccct catgagaatg tctgaaatat cacgcatcag ggttggggac ttggatcaga
      781 caggagacac agtgaccctg cacatcagtc acactaagac aatcaccaca gctgcgggcc
      841 ttgacaaagt gctctcccgg cgaaccacag cagtgctcaa tgactggctg gacgtcagtg
      901 ggctta
//
