LOCUS       sequence-79882-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the Dre-dependent Roxed-Cre gene under the control of 
            the Thy1.2 expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: Thy1.2-Roxed-Cre
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             162..182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    307..338
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    526..660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(563..582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     617..636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aagagagggg ctgaggtatt catcatgtgc tccgtggatc tcaagccctc aaggtaaatg
       61 gggacccacc tgtcctacca gctggctgac ctgtagcttt ccccaccaca gaatccaagt
      121 cggaactctt ggcacctaga ggatctcgac ccgccaccat gcccaagaag aagaggaagg
      181 tgacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg caggccttct
      241 ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc
      301 tgaacgtaac tttaaataat tggcattatt taaagttaat gtatgcgcta cgaagttatt
      361 cgcgatgaat aaatgaaagc ttgcagatct gcgactctag aggatctgcg actctagagg
      421 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
      481 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
      541 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      601 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
      661 tgcgactcta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      721 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      781 acttgtttat tgcagcttat aatggttaca aataaagcaa ta
//
