LOCUS       sequence-79899-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of the site-specific recombinase Dre and BFP under the
            control of the hGFAP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: hGFAP-NLS-HA-Dre-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(679..735)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tttgctaggg aggtcgcagt atctggccac tgccacctcg tgctgctcga cgtaggtctc
       61 gttgttggcc tccttgattc tttccagtct gtagtccaca tagtagacgc caggcatctt
      121 gaggttctta gcgggtttct tggatctata tgtggtcttg atgtttgcga tcagatggct
      181 cccgcccacg agcttcaggg ccatgtcgtt tctgccttcc aggccgccgt cagcggggta
      241 cagggtctcg gtgaaggcct cccagccgag tgttttcttc tgcatcacag ggccgttgga
      301 tgtgaagttc acccctctga tcttgacgtt gtagatgagg cagccgtcct ggaggctggt
      361 gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa
      421 gccctcaggg aaggactgct tgaagaagtc ggggatgccc tgggtgtggt tgatgaaggt
      481 cttgctgccg tagaggaagc tagtagccag gatgtcgaag gcgaagggga gagggccgcc
      541 ctcgaccacc ttgattctca tggtctgggt gccctcgtag ggcttgcctt cgccctcgga
      601 tgtgcacttg aagtgatggt tgtccacggt gccctccatg tacagcttca tgtgcatgtt
      661 ctccttaatc agctcgctgg gaccggggtt ttcttccacg tcgcctgctt gctttaacag
      721 agagaagttc gtggcgccgc tgccgctatc catcagtcga gaattggctc ccacggacac
      781 tccgcccctt ctgagatacc gcatcagtgt ttcaggcttc ttccatgtcc cttcctgcat
      841 aatctcgacc atagacacct gtttctcggc catgtctatt gcggcaccca ccctggcgga
      901 atggccggtc caggtcctat ac
//
