LOCUS       sequence-79901-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bxb1-responsive fluorescent reporter plasmid containing reverse 
            complementary ZsGreen flanked by Bxb1 attB/P recognition sites in 
            head-to-head orientation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-attBr-ZsGreen-attP
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..742
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTNNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     protein_bind    750..799
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 accaccgcgg tctcagtggt gtacggtaca aaccgccgcc accaccatgg cccagtccaa
       61 gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg tggacggcca
      121 caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc aggccatcaa
      181 cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt ccgccgcctt
      241 catgtacggc aaccgcgtgt tcaccgagta cccccaggac atcgtcgact acttcaagaa
      301 ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg gcgccgtgtg
      361 catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc acgagtccaa
      421 gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga ccaacaactg
      481 ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga agggcgacgt
      541 gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg acaccgtgta
      601 caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc acaagctgac
      661 ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc acgccatcgc
      721 ctccggctcc gccttgccct gaaccgtcag cccggatgat cctgacgacg gagaccgccg
      781 tcgtcgacaa gccggccgat taattaagga tctgtaggaa aa
//
