LOCUS       sequence-79920-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSV40mod-luc2 donor plasmid for MPRA library construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
  TITLE     Massively parallel reporter assays in cultured Mammalian cells.
  JOURNAL   J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
  PUBMED    25177895
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-08-17"; volume: "(90). doi"; pages: " 
            10.3791/51719"
COMMENT     Sequence Label: pMPRAdonor3
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..50
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     42..59
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     43..59
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        129..325
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      176..311
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      349..358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
       61 aattcgagct cggtacctga gctcgctagc ctcgaggata tcaagatctg gcctcggcgg
      121 ccaagctttg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
      181 gcccctaact ccgcccagtt ccgcccattc tccgccccat cgctgactaa ttttttttat
      241 ttatgcagag gccgaagtcg cctcggcctc tgagctattc cagaagtagt gaggaggctt
      301 ttttggaggc ctaggctttt gcaaaggcaa tccggtactg ttggtaaagc caccatggaa
      361 gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga cgggaccgcc
      421 ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac catcgccttt
      481 accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat gagcgttcgg
      541 ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc
      601 gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg
      661 gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg catcagccag
      721 cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag
      781 ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca gggcttccaa
      841 agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta cgacttcgtg
      901 cccgagagct tcgaccggga
//
