LOCUS       sequence-79923-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  minP-luc2 donor plasmid for MPRA library construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
  TITLE     Massively parallel reporter assays in cultured Mammalian cells.
  JOURNAL   J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
  PUBMED    25177895
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-08-17"; volume: "(90). doi"; pages: " 
            10.3791/51719"
COMMENT     Sequence Label: pMPRAdonor2
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..50
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     42..59
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     43..59
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        135..166
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      193..202
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
       61 aattcgagct cggtacctga gctcgctagc ctcgaggata tcaagatctg gcctcggcgg
      121 ccaagcttag acactagagg gtatataatg gaagctcgac ttccagcttg gcaatccggt
      181 actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc
      241 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
      301 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
      361 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
      421 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
      481 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
      541 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
      601 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
      661 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
      721 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
      781 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
      841 gcttgtgtcc gattcagtca tgcccgcgac ccc
//
