LOCUS       sequence-79927-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kanamycin resistant; contains qtag20 and constitutively expresses 
            reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMCKq20-T38S38-0X
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..25
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ctatcaactt tgtatagaaa agttgcagct ggctagcgag tcatgaggtc accatggtca
       61 ttaggagccg ctctcgcact tcagctgctt ctccaagccg cagatgatca gctccaggcc
      121 gaacaagaag gccggctcgg cgccctggtg gtcgaacagc tcgatggcct ggcgcagcaa
      181 cggcggcatg ctgtcggtgg tcggggtctc gcgctcctcc ttggcggccg tgtgctcctg
      241 gtcctccaac acgcagccca gggtgaagtg gcccacggcc gacagggcgt acaacgcgtt
      301 ctccagcgag aagccgttct cggtcatgaa gcgcagctgg gtctccacgg tgtcgtactg
      361 cttctcgtcc gggcgggtgc ccaggtgcac cttggcgccg tcgcggtagc gcagcagcgc
      421 gcggcggaac gacatggcgt tgttgcgcag gaacgactgc cacgactcgc cggccgccgg
      481 cagcgagtag tcgtggtggc gcgccaggat ctccaccgcc agggcgtcca gcagggcccg
      541 cttgttcttg acgtgccagt acagggtctt ctgcgcgacg cccagcttct gggccagctt
      601 gcgggtggtc acgccctcga tgccgacctc gttgcccagg gccagggcgg agttgatgac
      661 cttggacttg tccaggcggg acattgcgaa gtgattcctc ctgatggtac cctaggtcag
      721 atcaacggcc tggtcgacaa gggcaatacg gtgatcgtca tcgaacataa cctggacgtg
      781 atcggccgtc aactcgttga ggtaccggtc ggattcaaat ggttcgtcga cggtctgatc
      841 ccgcggtgtg caccgatgcc ctcacggttc cagcggtcga ccaggtcctc gccgtga
//
