LOCUS       sequence-79929-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kanamycin resistant; contains qtag20 and constitutively expresses 
            reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMCKq20-T38S38-0X
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(164..182)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(165..182)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    245..265
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    271..295
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(415..439)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 catgctccat cagcagcaag aacgccatac tatagcatgg tatcggcgcg ggacggcagc
       61 ctcttgaaga agttgcaagc ttgaacacca gatggacacg ctccaggact actacatggt
      121 tgcgcggcac gtaccgatgc cagctaggcc atcgaattcc acttctatag tgtcacctaa
      181 atcaccggtt ataaaaaatt tttaaaataa aaaaggggac ctctagggtc ccctgcagct
      241 taagcaacta tgtataataa agttgacgcg tccactttgt acaagaaagc tgggttcgcg
      301 cctacctgct caaagagagc ctgcggcatg tgttttcggt caagggcgag gaaggtaaac
      361 aggccctgga ccggtggatc tcctgggccc agcgctgtcg catcccggta ttcgagcctg
      421 cttttttgta caaacttgaa tattggatca cgccgcgagc gtgtcggtgt tcgaagacct
      481 gggaatccag caggcgccca acagcaccgt ctgctccgtc ttctccagaa aggcgactac
      541 gcggtcctcc tcgtcggtgg gtacgcagcc gaatgcccgc gggtcgccca cccgcaccag
      601 ttgcagcgtg acatcggctc gattgcttcg gtggaagtcc agcagttggg ccagatccgc
      661 gagggccccc cgtgaacatc gtcgtggtaa ccagcgtgtc ggctttggct gtggccgtgg
      721 tgcattcggt ggcgtttgcg atcaaggggc gtcacggcga ggacctggtc gaccgctgga
      781 accgtgaggg catcggtgca caccgcggga tcagaccgtc gacgaaccat ttgaatccga
      841 ccggtacctc aacgagttga cggccgatca cgtccaggtt atgttcgatg acgatcaccg
      901 tattgccctt gtcga
//
