LOCUS       sequence-79934-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length RAMA 
            ectodomain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: RAMA-bio-His
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     507..526
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      549..558
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcctca ctctcttccg catcgctgtc tgcgagggcc
       61 agctgttggg ctcgcggttg aggacaaact cttcgcggtc tttccagtac tcttggatcg
      121 gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg acctgagcga gtccgcatcg
      181 accggatcgg aaaacctctc gagaaaggcg tctaaccagt cacagtcgca aggtaggctg
      241 agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt ttctggcgga ggtgctgctg
      301 atgatgtaat taaagtaggc ggtcttgaga cggcggatgg tcgaggtgag gtgtggcagg
      361 cttgagatcc agctgttggg gtgagtactc cctctcaaaa gcgggcatta cttctgcgct
      421 aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg atctggccat
      481 acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt
      541 ccaagtttgc caccatggag tttcagaccc aggtactcat gtccctgctg ctctgcatgt
      601 ctggtgcggc cgcatacctg gaacagatca agaacggcat cagcggccac tacaccgagg
      661 accacaacat caagaacaac aactgcatca gcttcagcga ctacgagcgg agcatcaaga
      721 atttcgccat cagcagccac gccgagaaca actacgacaa catcatcaac gagtacaaga
      781 agatcaagga catcaacaac aatatcaaca tcctgagcag cgtgcaccgg aagggccgga
      841 tcctgtacga cagcttcctg gaaatcaaca agctcgagaa cgacaagaaa gagaa
//
