LOCUS       sequence-79938-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, 
            Tabar V, Mo Q, Studer L, Sadelain M
  TITLE     Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and 
            c-Myc expression for efficient human iPSC induction and 
            differentiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
  PUBMED    19549847
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Aug 4. 106(31):12759-64."
COMMENT     Sequence Label: pLM-mCherry-Klf4
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(49..68)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(50..68)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(78..95)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(78..94)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(87..109)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      236..691
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(323..342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     533..554
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
       61 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      121 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      181 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
      241 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      301 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      361 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      421 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      481 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      541 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
      601 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
      661 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
      721 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      781 aaccctgata aatgcttcaa taatattgaa
//
