LOCUS       sequence-79940-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3C8X
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     81..143
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             156..173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
       61 gcggatctcg gtccgaaacc atgaaattct tagtcaacgt tgcccttgtt tttatggtcg
      121 tatacatttc ttacatctat gcggccgctc cggaacatca ccatcaccat cacgattacg
      181 atatcccaac gaccgaaaac ctgtattttc agggcgccat ggatgcggcg cagggcaagg
      241 aagtggtact gctggacttt gctgcagctg gaggggagct cggctggctc acacacccgt
      301 atggcaaagg gtgggacctg atgcagaaca tcatgaatga catgccgatc tacatgtact
      361 ccgtgtgcaa cgtgatgtct ggcgaccagg acaactggct ccgcaccaac tgggtgtacc
      421 gaggagaggc tgagcgtatc ttcattgagc tcaagtttac tgtacgtgac tgcaacagct
      481 tccctggtgg cgccagctcc tgcaaggaga ctttcaacct ctactatgcc gagtcggacc
      541 tggactacgg caccaacttc cagaagcgcc tgttcaccaa gattgacacc attgcgcccg
      601 atgagatcac cgtcagcagc gacttcgagg cacgccacgt gaagctgaac gtggaggagc
      661 gctccgtggg gccgctcacc cgcaaaggct tctacctggc cttccaggat atcggtgcct
      721 gtgtggcgct gctctccgtc cgtgtctact acaagaagtg cccctgatga agcttgtcga
      781 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
      841 aa
//
