LOCUS       sequence-79946-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4GLM
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      320..367
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 tttccgagca cttcaccaac aaggaccata gattatgaaa atcgaagaac accatcacca
       61 tcaccattcc agcggtcgtg aaaatctgta cttccaaggt ggcgcggccc agccggccat
      121 ggcccagggc gggctgacct atggtgttgc cctgtatcgt tttcaagccc tggaaccgaa
      181 cgaactggat ttcgaagttg gtgacaaaat ccgtattctg gccaccttag aagatggttg
      241 gctggaaggt tctctgaaag gtcgtaccgg tatctttccg tatcgtttcg ttaaactgtg
      301 cccggcggcc gcataataaa ccgatacaat taaaggctcc ttttggagcc ttttttttgg
      361 agattttcaa cgtgaaaaaa ttattattcg caattccttt agttgttcct ttctattctc
      421 actccgctga aactgttgaa agttgtttag caaaacccca tacagaaaat tcatttacta
      481 acgtctggaa agacgacaaa actttagatc gttacgctaa ctatgagggc tgtctgtgga
      541 atgctacagg cgttgtagtt tgtactggtg acgaaactca gtgtctagct agagtggcgg
      601 tggctctggt tccggtgatt ttgattatga aaagatggca aacgctaata agggggctat
      661 gaccgaaaat gccgatgaaa acgcgctaca gtctgacgct aaaggcaaac ttgattctgt
      721 cgctactgat tacggtgctg ctatcgatgg tttcattggt gacgtttccg gccttgctaa
      781 tggtaatggt gctactggtg attttgctgg ctctaattcc caaatggctc aagtcggtga
      841 cggtgataat tcacctttaa tgaataattt ccgtcaatat ttaccttccc tccctcaatc
      901 ggttgaatgt c
//
