LOCUS       sequence-79947-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4HC4
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             87..104
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             117..137
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg
       61 gcgcggatct cggtccgaaa accatgcatc atcatcatca tcacagcagc ggcagagaaa
      121 acttgtattt ccagggcatg tcgcagccca agaaaagaaa gcttgagtcg gggggcggcg
      181 gcgaaggagg ggagggaact gaagaggaag atggcgcgga gcgggaggcg gccctggagc
      241 gaccccggag gactaagcgg gaacgggacc agctgtacta cgagtgctac tcggacgttt
      301 cggtccacga ggagatgatc gcggaccgcg tccgcaccga tgcctaccgc ctgggtatcc
      361 ttcggaactg ggcagcactg cgaggcaaga cggtactgga cgtgggcgcg ggcaccggca
      421 ttctgagcat cttctgtgcc caggccgggg cccggcgcgt gtacgcggta gaggccagcg
      481 ccatctggca acaggcccgg gaggtggtgc ggttcaacgg gctggaggac cgggtgcacg
      541 tcctgccggg accagtggag actgtagagt tgccggaaca ggtggatgcc atcgtgagcg
      601 agtggatggg ctacggactc ctgcacgagt ccatgctgag ctccgtcctc cacgcgcgaa
      661 ccaagtggct gaaggagggc ggtcttctcc tgccggcctc cgccgagctc ttcatagtcc
      721 ccatcagcga ccagatgctg gaatggcgcc tgggcttctg gagccaggtg aagcagcact
      781 atggtgtgga catgagctgc ctggagggct tcgccacgcg ctgtctcatg ggccactcgg
      841 agatcgttgt gca
//
