LOCUS       sequence-79951-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4IIM
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      313..360
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 cgagcacttc accaacaagg accatagatt atgaaaatcg aagaacacca tcaccatcac
       61 cattccagcg gtcgtgaaaa tctgtacttc caaggtggcg cggcccagcc ggccatggcc
      121 cagggcgcgc tgctgcaagc acaagcattg tatccatggc gtgccaaaaa agataaccat
      181 ctgaacttta acaaaaacga tgtcattacc gtcctggaac aacaagatat gtggtggttt
      241 ggtgaagttc agggtcagaa aggctggttt ccgaaatcct atgttaaact gatttccgcg
      301 gccgcataat aaaccgatac aattaaaggc tccttttgga gccttttttt tggagatttt
      361 caacgtgaaa aaattattat tcgcaattcc tttagttgtt cctttctatt ctcactccgc
      421 tgaaactgtt gaaagttgtt tagcaaaacc ccatacagaa aattcattta ctaacgtctg
      481 gaaagacgac aaaactttag atcgttacgc taactatgag ggctgtctgt ggaatgctac
      541 aggcgttgta gtttgtactg gtgacgaaac tcagtgtcta gctagagtgg cggtggctct
      601 ggttccggtg attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa
      661 aatgccgatg aaaacgcgct acagtctgac gctaaaggca aacttgattc tgtcgctact
      721 gattacggtg ctgctatcga tggtttcatt ggtgacgttt ccggccttgc taatggtaat
      781 ggtgctactg gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat
      841 aattcacctt taatgaataa tttccgtcaa tatttacctt ccctccctca atcggttgaa
      901 tg
//
