LOCUS       sequence-79952-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4IGZ
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      367..414
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 cgagcacttc accaacaagg accatagatt atgaaaatcg aagaacacca tcaccatcac
       61 cattccagcg gtcgtgaaaa tctgtacttc caaggtggcg cggcccagcc ggccatggcc
      121 cagggcgcgc tgctgccagc aaaagccgtt tacgatttca aagcccagac ctccaaagaa
      181 ctgtccttta aaaaaggtga taccgtttac attctgcgta aaattgatca gaactggtat
      241 gaaggtgaac atcatggtcg tgttggtatc ttcccgattt cctacgtcga aaaactgacc
      301 ggatccgcgg ccgcactgcg caccggcgaa gcgtatctgc gctatgtgga tgcggccgca
      361 taataaaccg atacaattaa aggctccttt tggagccttt tttttggaga ttttcaacgt
      421 gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc tattctcact ccgctgaaac
      481 tgttgaaagt tgtttagcaa aaccccatac agaaaattca tttactaacg tctggaaaga
      541 cgacaaaact ttagatcgtt acgctaacta tgagggctgt ctgtggaatg ctacaggcgt
      601 tgtagtttgt actggtgacg aaactcagtg tctagctaga gtggcggtgg ctctggttcc
      661 ggtgattttg attatgaaaa gatggcaaac gctaataagg gggctatgac cgaaaatgcc
      721 gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc tactgattac
      781 ggtgctgcta tcgatggttt cattggtgac gtttccggcc ttgctaatgg taatggtgct
      841 actggtgatt ttgctggctc taattcccaa atggctc
//
