LOCUS       sequence-79953-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4I79
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             121..138
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
       61 gcggatctcg gtccgaaaac catgggcagc agccatcatc atcatcatca cagcagcggc
      121 ctggttccgc gtggtagtat ggaggaaatt tatgcgaagt ttgtgtccca gaaaatcagc
      181 aaaacccgct ggcgaccgct gcctccggga agtttacaga ccgcggagac gttcgctaca
      241 ggatcttggg acaatgagga aaattatatt tcactgtggt ctattggaga ttttggaaac
      301 ttggactctg atggagggtt tgaaggagac catcagttat tgtgtgatat cagacaccat
      361 ggtgatgtaa tggatttaca gttttttgac caggaaagaa ttgtcgctgc ttcatcaaca
      421 ggatgtgtaa cagttttcct tcaccatcca aataaccaga ctctgtcagt caaccagcag
      481 tggactacag ctcactacca cacaggccct ggcagtcctt cctatagcag tgcaccatgt
      541 acaggtgttg tgtgcaacaa cccagaaatc gttacagttg gagaggatgg tcgaataaat
      601 ctcttcagag ctgatcacaa ggaagctgta agaaccatag acaatgcaga tagtagtaca
      661 ctccatgctg taacctttct tcgaactcct gagattctta ctgtaaattc aattggacag
      721 ttgaaaatat gggatttcag acaacaagga aatgagcctt ctcagatatt gtcactgact
      781 ggtgaccgag tg
//
