LOCUS       sequence-79957-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4EDU
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             785..802
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(855..873)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tagaataatt ttgtttaact ttaagaagga gatataccat gcatcatcat catcatcaca
       61 gcagcggcag agaaaacttg tatttccagg gctcaagtac atcttctgta caaagggatg
      121 atttccactg ggaggagtat ttgaaagaga ctgggtctat aagtgctcct tcagagtgct
      181 tccgtcagtc tcagattcca cctgtgaatg atttcaaagt tggtatgaaa ttggaagccc
      241 gtgaccctcg caatgccact tcagtatgta ttgctacggt tattggaatt actggggcca
      301 ggttacggtt acgactggat ggtagtgaca acagaaatga tttttggagg cttgtcgatt
      361 ccccagatat acaacctgtt gggacatgtg aaaaggaagg agacttactt caacctccac
      421 tagggtacca gatgaataca tcctcctggc cgatgttcct cttaaagaca ctaaatgggt
      481 ctgaaatggc atctgccaca ttatttaaga aggaaccacc aaagccccca ctaaataatt
      541 ttaaagtggg gatgaaactg gaagctattg acaaaaagaa cccgtatctc atctgtcctg
      601 cgaccattgg agatgttaaa ggggatgaag ttcatatcac atttgatggc tggagtggag
      661 cttttgatta ctggtgcaag tatgattctc gagatatttt cccagctggg tggtgtcgcc
      721 tgacaggaga tgtattacaa cccccaggaa ctagttgatg acgaagcttg cggccgcact
      781 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      841 gttggctgct gccaccgctg agcaataact agca
//
