LOCUS       sequence-79960-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3DAX
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(147..152,153..170,171..177)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    185..201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(256..261,262..277,278..284)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    292..308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(351..356,357..372,373..379)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    387..403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc gatggtgtcc
       61 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt
      121 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
      181 ggaattgtga gcggataaca atttcacaca ggaaacagga tcgatccatc gatgagctta
      241 ctccccatcc ccctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg
      301 gataacaatt tcacacagga aacaggatca gcttactccc catccccctg ttgacaatta
      361 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
      421 gatccatcga tgcttaggag gtcatatggc taagaaaacg agctctagga gaaggcaaac
      481 gggtgaacca cctctagaga atggattaat tccatacctg ggctgtgctc tgcaatttgg
      541 tgccaatcct cttgagttcc tcagagcaaa tcaaaggaaa catggtcatg tttttacctg
      601 caaactaatg ggaaaatatg tccatttcat cacaaatccc ttgtcatacc ataaggtgtt
      661 gtgccacgga aaatattttg attggaaaaa atttcacttt
//
