LOCUS       sequence-79961-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  TITLE     pME-hygro-SNAP-FLAG-CD59
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pME-hygro-SNAP-FLAG-CD59
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(136..465)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      150..285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(204..223)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     450..470
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(633..654)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
       61 cgtcgcggtg agttcaggct ttttcatcac gtgctgatca gatccgaaaa tggatataca
      121 agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
      181 gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
      241 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
      301 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
      361 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
      421 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagaattag
      481 cttggtggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
      541 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
      601 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
      661 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
      721 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
      781 accctaa
//
