LOCUS       sequence-79977-e        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with attL5 and attL2 sites flanking a CmR and 
            ccdB gene. In our hands, the multisite reaction still proceeds 
            efficiently. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pNJB80
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(133..232)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(249..268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(250..268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(273..289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(273..289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcaggtcga cgacccagct ttcttgtaca aagttggcat tataaaaaat aattgctcat
      181 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
      241 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
      301 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc
      361 tctggaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
      421 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
      481 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      541 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
      601 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
      661 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
      721 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      781 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      841 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      901 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      961 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     1021 atccagctaa gcgcgaactg c
//
