LOCUS       sequence-79978-e         314 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with attL5 and attL2 sites flanking a CmR and 
            ccdB gene. In our hands, the multisite reaction still proceeds 
            efficiently. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pNJB80
FEATURES             Location/Qualifiers
     source          1..314
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(97..103,104..121,122..127))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..255)
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(270..287)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(270..286)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(279..301)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
       61 ctgaaaatct cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa
      121 gtgtaaagcc tggggtgcct aatgcggccg cgagctcggc aacttttgta tacaaagttg
      181 gcattataaa aaagcattgc tcatcaattt gttgcaacga acaggtcact atcagtcaaa
      241 ataaaatcat tattgggccc gagcttaaga ctggccgtcg ttttacaacg tcgtgactgg
      301 gaaaacatcc atgc
//
