LOCUS       sequence-79981-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Guo X, Zhang T, Hu Z, Zhang Y, Shi Z, Wang Q, Cui Y, Wang F, Zhao H,
            Chen Y
  TITLE     Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.
  JOURNAL   Development. 2014 Jan 8.
  PUBMED    24401372
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2014 Jan 8."
COMMENT     Sequence Label: pCS2-3xFLAG-NLS-SpCas9-NLS
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttcgctcag gccgcctctc tcggccttgg tcagattgtc gaactttctc tgggtaatca
       61 gcttggcgtt cagcagctgc cgccagtagt tcttcatctt cttcacgacc tcttcggagg
      121 gcacgttgtc gctcttgccc cggttcttgt cgcttctggt cagcaccttg ttgtcgatgg
      181 agtcgtcctt cagaaagctc tgaggcacga tatggtccac atcgtagtcg gacagccggt
      241 tgatgtccag ttcctggtcc acgtacatat cccgcccatt ctgcaggtag tacaggtaca
      301 gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc tttcaggatc tggctgccca
      361 gctctttgat gccctcttcg atccgcttca ttctctcgcg gctgttcttc tgtcccttct
      421 gggtggtctg gttctctctg gccatttcga tcacgatgtt ctcgggcttg tgccggccca
      481 tcactttcac gagctcgtcc accaccttca ctgtctgcag gatgcccttc ttaatggcgg
      541 ggctgccggc cagattggca atgtgctcgt gcaggctatc gccctggccg gacacctggg
      601 ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc atgaagtttc
      661 tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt cttgccggac tgcttgtccc
      721 ggatgccgtt gatcagcttc cggctcagcc tgccccagcc ggtgtatctc cgccgcttca
      781 gctgcttcat cactttgtcg tcgaacaggt gggcataggt tttcagccgt tcctcgatca
      841 tctctctgtc ctcaaacagt gtcagggtca gcacgatatc ttccagaatg tcctcgtttt
      901 cctcattgtc caggaagtcc ttgtccttga taattttcag cagatcgtgg tatgtgccca
      961 gggaggcgtt gaaccgatct tccacgccgg agatttccac ggagtc
//
