LOCUS       sequence-79982-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 nickase optimized for nuclear import..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins 
            A, Iyer V, Huang X, Skarnes WC
  TITLE     Efficient genome modification by CRISPR-Cas9 nickase with minimal 
            off-target effects.
  JOURNAL   Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857.
  PUBMED    24584192
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857."
COMMENT     Sequence Label: pST1374-N-NLS-flag-linker-Cas9-H840A
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaagggcc cctagtcgcc gccgagctga gacaggtcga tgcgagtctc
      121 gtacagtccg gtaattgact ggtgtatcag ggtggcatcg agaacttctt tggtagaggt
      181 gtaccgcttc ctgtcaatag ttgtatcgaa atacttgaag gcagcaggag cgcccagatt
      241 agtcagagta aagaggtgga taatattctc tgcttgttcg cgaattggct tgtccctgtg
      301 cttattatat gcgctcagca ctttatcgag gtttgcatcg gccagaataa cccgcttgct
      361 gaactcgcta atctgttcaa tgatttcgtc caggtagtgt ttatgttgct caacaaagag
      421 ttgcttctgc tcattgtctt cagggctacc tttgagtttc tcgtagtggg aggccagata
      481 caggaagttc acgtatttgg agggcagagc cagctcgttt cctttctgca gctctccggc
      541 ggaggccagc atccgcttcc taccattctc cagctcaaag agagagtact tgggcagttt
      601 gatgatgaga tctttcttca cttctttata gcccttagct tccaggaaat cgattggatt
      661 cttctcgaag ctggatctct ccataatagt aattccgagc agctccttaa cagacttgag
      721 tttcttggac ttgcctttct ccacttttgc cacgaccaga acggaataag ccactgtagg
      781 ggaatcgaaa ccgccatact tctttgggtc ccaatctttc ttcctggcga tcagcttgtc
      841 agagttccgc tttggcagga tgctctcctt tgagaatccg ccggtctgca cttcggtctt
      901 cttcacgata ttgacttgtg gcatggacag caccttccgc acagt
//
