LOCUS       sequence-79984-e        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by 
            rice snoRNA U3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Xie K, Yang Y
  TITLE     RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
  JOURNAL   Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 
            Aug 17.
  PUBMED    23956122
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/mp/sst119";
            journalName: "Mol Plant"; date: "2013-11"; volume: "6"; issue: "6"; 
            pages: "1975-83"
COMMENT     Sequence Label: pRGE31
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..397
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        414..489
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tgattacgcc aagcttaagg aatctttaaa catacgaaca gatcacttaa agttcttctg
       61 aagcaactta aagttatcag gcatgcatgg atcttggagg aatcagatgt gcagtcaggg
      121 accatagcac aagacaggcg tcttctactg gtgctaccag caaatgctgg aagccgggaa
      181 cactgggtac gttggaaacc acgtgatgtg aagaagtaag ataaactgta ggagaaaagc
      241 atttcgtagt gggccatgaa gcctttcagg acatgtattg cagtatgggc cggcccatta
      301 cgcaattgga cgacaacaaa gactagtatt agtaccacct cggctatcca catagatcaa
      361 agctgattta aaagagttgt gcagatgatc cgtggcagga gaccgaggtc tcggttttag
      421 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      481 agtcggtgct tttttgtttt agagctagaa atagcaagtt aaaataaggc tagtccgttt
      541 ttagcgcgtg catgcctgca ggtccccaga ttagcctttt caatttcaga aagaatgcta
      601 acccacagat ggttagagag gcttacgcag cagcactcat caagacgatc tacccgagca
      661 ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca
      721 ggactaactg catcaagaac acagagaaag atatatttct caagatcaga agtactattc
      781 cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag attggagtct
      841 ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa atagaggacc
      901 taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg
      961 acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata
     1021 tcaaagatac agtctcagaa gaccaaag
//
