LOCUS       sequence-79986-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gateway adapted mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang 
            X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, 
            Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz
            G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, 
            Meyerson M
  TITLE     Functional analysis of receptor tyrosine kinase mutations in lung 
            cancer identifies oncogenic extracellular domain mutations of ERBB2.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug
            20.
  PUBMED    22908275
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-4"; volume: "109(36)"; pages: 
            "14476-81. Epub 2012 Aug 20"
COMMENT     Sequence Label: pBABEpuro-gateway
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(140..264)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(292..708)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(308..324)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(340..362)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
      121 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
      181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      241 ttcagctttt ttgtacaaac ttgtgatgta ccaccacact gggatccggc cggcgcctag
      301 agaaggagtg agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag
      361 ggggagagac ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt
      421 gacccaggga ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg ggacctggtc
      481 tgggtgtcca tgcgggccag gtgaaaagac cttgatctta acctgggtga tgaggtctcg
      541 gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt ggccattctg cagagcagaa
      601 ggtaacccaa cgtctcttct tgacatctac cgactggttg tgagcgatcc gctcgacatc
      661 tttccagtga cctaaggtca aacttaaggg agtggtaaca gtctggccct aattttcaga
      721 caaatacaga aacacagtca gacagagaca acacagaacg atgctgcagc gctgcagcag
      781 acaagacgcg cggcttcggt tccaaaccga aagcaaaaat tcagacgga
//
