LOCUS       sequence-79987-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into 
            plants by agrobacterium mediated transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Xie K, Yang Y
  TITLE     RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
  JOURNAL   Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 
            Aug 17.
  PUBMED    23956122
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/mp/sst119";
            journalName: "Mol Plant"; date: "2013-11"; volume: "6"; issue: "6"; 
            pages: "1975-83"
COMMENT     Sequence Label: pRGEB31
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(266..289)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    300..324
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(357..404)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
       61 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
      121 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
      181 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
      241 gatcggggaa attcgagctc taagccttgt catcgtcatc cttgtagtcg ctgttatcaa
      301 ccactttgta caagaaagct gggtctagat atctcgagtg cggccgcgaa ttcttacttt
      361 ttcttttttg cctggccggc ctttttcgtg gccgccggcc ttttgtcgcc tcccagctga
      421 gacaggtcga tccgtgtctc gtacaggccg gtgatgctct ggtggatcag ggtggcgtcc
      481 agcacctctt tggtgctggt gtacctcttc cggtcgatgg tggtgtcaaa gtacttgaag
      541 gcggcagggg ctcccagatt ggtcagggta aacaggtgga tgatattctc ggcctgctct
      601 ctgatgggct tatcccggtg cttgttgtag gcggacagca ctttgtccag attagcgtcg
      661 gccaggatca ctctcttgga gaactcgctg atctgctcga tgatctcgtc caggtagtgc
      721 ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct cgggggagcc cttca
//
