LOCUS       sequence-79990-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplies the selection marker (eCFP) and IRES with flanked by AttR2 
            and AttL3 sites for Gateway cloning recombination reaction. (This 
            corresponds to a module 3 plasmid)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R2-eCFP-L3   #BGV202
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    36..131
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(236..257)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     585..604
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tatagggcga attggggatc gaagttaaca ctagtaaata atgattttat tttgactgat
       61 agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta
      121 taataaagtt gcccgggggc gcgcccaccc ttaactcgag cctaggagat ccgctgaggg
      181 aaagtctaga ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      241 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
      301 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      361 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttgg
      421 ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtggctg atgtagttgt
      481 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      541 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      601 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      661 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccc caggtcaggg
      721 tggtcacgag ggtgg
//
