LOCUS       sequence-79998-e        1847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T
  TITLE     Reversible dimerization of Aequorea victoria fluorescent proteins 
            increases the dynamic range of FRET-based indicators.
  JOURNAL   ACS Chem Biol. 2010 Feb 19;5(2):215-22. doi: 10.1021/cb900263z.
  PUBMED    20047338
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2010-02-19- 19"; volume: "5"; issue: "2"; 
            pages: "215-22"
COMMENT     Sequence Label: Ateam1.03-nD/nA/pRSETB
FEATURES             Location/Qualifiers
     source          1..1847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..526)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             1106..1840
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 ctcgagtatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca
      481 gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg gcggcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc catcgatatg aaaactgtga aagtgaatat
      721 aacaacccct gatgggccag tctacgacgc tgatatcgag atggtgtccg tgcgggccga
      781 gagtggtgat ctcggcatcc tccccggtca cattcccaca aaggccccac tgaagatcgg
      841 agctgtgcgg ctgaagaagg acggccaaac cgagatggtc gcagtctcag gcggcactgt
      901 tgaagtgcgg cctgaccacg ttaccattaa tgctcaagcc gctgaaacag ccgaaggaat
      961 cgacaaagag agagcagaag ccgcaagaca gagggcccag gagcggctga actctcaatc
     1021 cgatgacacc gatattcgcc gggccgagct ggcactgcag agggccctga acagactgga
     1081 cgtggctggg aaggcaaacg aattcatgga cggcggcgtg cagctcgccg accactacca
     1141 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     1201 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1261 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggtg gcagcggtgg
     1321 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1381 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1441 cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac
     1501 cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
     1561 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1621 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1681 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1741 caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa
     1801 cggcatcaag gccaacttca agatccgcca caacatcgag taagctt
//
