LOCUS       sequence-80000-e        1971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ 
            indicator with Kd = 15 nM, in Dictyostelium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura
            T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T
  TITLE     Spontaneous network activity visualized by ultrasensitive Ca(2+) 
            indicators, yellow Cameleon-Nano.
  JOURNAL   Nat Methods. 2010 Sep;7(9):729-32. doi: 10.1038/nmeth.1488. Epub 
            2010 Aug 8.
  PUBMED    20693999
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1488"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "729-32"
COMMENT     Sequence Label: Yellow Cameleon-Nano15/pBig
FEATURES             Location/Qualifiers
     source          1..1971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1150..1227
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atggttagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
       61 ggtgatgtta atgggcacag attttctgtc agtggagagg gtgaaggtga tgcaacatac
      121 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      181 cttgtcacta ctttaacatg gggtgttcaa tgcttttcaa gatacccaga tcatatgaaa
      241 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      301 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      361 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      421 aaattggaat acaactatat ttcacacaat gtatacatca cagcagacaa acaaaagaat
      481 ggaatcaaag caaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      541 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      601 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      661 cttcttgagt ttgtaacagc tgctcgcatg catgaccaac tgacagaaga gcagattgca
      721 gagttcaaag aagccttctc attattcgac aaggatgggg acggcaccat caccacaaag
      781 gaacttggca ccgttatgag gtcgcttgga caaaacccaa cggaagcaga attgcaggat
      841 atgatcaatg aagtcgatgc tgatggcaat ggaacgattt actttcctga atttcttact
      901 atgatggcta gaaaaatgaa ggacacagac agcgaagagg aaatccgaga agcattccgt
      961 gtttttgaca aggatgggaa cggctacatc agcgctgctg aattacgtca cgtcatgaca
     1021 aacctcgggg agaagttaac agatgaagaa gttgatgaaa tgataaggga agcagatatc
     1081 gatggtgatg gccaagtaaa ctatgaagag tttgtacaaa tgatgacagc aaagggggga
     1141 ggtggctcca agaggcgctg gaagaaaaac ttcattgccg tcagcgctgc caaccggttc
     1201 aagaagatct ccagctccgg ggcactggag ctgatggatg gaggtgttca actagcagac
     1261 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1321 ctgtcctatc aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1381 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaaggtggc
     1441 agcggtggca tggttagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1501 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1561 gcaacatacg gaaaacttac ccttaaatta atttgcacta ctggaaaact acctgttcca
     1621 tggccaacac ttgtcactac tttaggttat ggtcttcaat gctttgcgag atacccagat
     1681 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1741 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1801 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1861 cttggacaca aattggaata caactataac tcacacaatg tatacatcac agcagacaaa
     1921 caaaagaatg gaatcaaagc aaacttcaaa attagacaca acattgaata a
//
