LOCUS       sequence-80005-e        2206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ 
            indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Saito K, Hatsugai N, Horikawa K, Kobayashi K, Matsu-Ura T, Mikoshiba
            K, Nagai T
  TITLE     Auto-luminescent genetically-encoded ratiometric indicator for 
            real-time Ca2+ imaging at the single cell level.
  JOURNAL   PLoS One. 2010 Apr 1;5(4):e9935. doi: 10.1371/journal.pone.0009935.
  PUBMED    20376337
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-04-1"; volume: "5(4):e9935. doi"; pages: " 
            10.1371/journal.pone.0009935"
COMMENT     Sequence Label: BRAC/pcDNA3
FEATURES             Location/Qualifiers
     source          1..2206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..724)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(53..74)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(314..333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1181..1258
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 ggatccgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
      481 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      721 caagcgcatg catgaccaac tgacagaaga gcagattgca gagttcaaag aagccttctc
      781 attattcgac aaggatgggg acggcaccat caccacaaag gaacttggca ccgttatgag
      841 gtcgcttgga caaaacccaa cggaagcaga attgcaggat atgatcaatg aagtcgatgc
      901 tgatggcaat ggaacgattt actttcctga atttcttact atgatggcta gaaaaatgaa
      961 ggacacagac agcgaagagg aaatccgaga agcattccgt gtttttgaca aggatgggaa
     1021 cggctacatc agcgctgctc agttacgtca cgtcatgaca aacctcgggg agaagttaac
     1081 agatgaagaa gttgatgaaa tgataaggga agcagatatc gatggtgatg gccaagtaaa
     1141 ctatgaagag tttgtacaaa tgatgacagc aaaggggggg aagaggcgct ggaagaaaaa
     1201 cttcattgcc gtcagcgctg ccaaccggtt caagaagatc tccagctccg gggcactgga
     1261 gctcatgacc agcaaggtgt acgaccccga gcagaggaag aggatgatca ccggccccca
     1321 gtggtgggcc aggtgcaagc agatgaacgt gctggacagc ttcatcaact actacgacag
     1381 cgagaagcac gccgagaacg ccgtgatctt cctgcacggc aacgccacta gcagctacct
     1441 gtggaggcac gtggtgcccc acatcgagcc cgtggccagg tgcatcatcc ccgatctgat
     1501 cggcatgggc aagagcggca agagcggcaa cggcagctac aggctgctgg accactacaa
     1561 gtacctgacc gcctggttcg agcttctgaa cctgcccaag aagatcatct tcgtgggcca
     1621 cgactggggc gccgccctgg ccttccacta cgcctacgag caccaggaca ggatcaaggc
     1681 catcgtgcac atggagagcg tggtggacgt gatcgagagc tgggacgagt ggccagacat
     1741 cgaggaggac atcgccctga tcaagagcga ggagggcgag aagatggtgc tggagaacaa
     1801 cttcttcgtg gagaccgtgc tgcccagcaa gatcatgaga aagctggagc ccgaggagtt
     1861 cgccgcctac ctggagccct tcaaggagaa gggcgaggtg agaagaccca ccctgagctg
     1921 gcccagagag atccccctgg tgaagggcgg caagcccgac gtggtgcaga tcgtgagaaa
     1981 ctacaacgcc tacctgagag ccagcgacga cctgcccaag ctgttcatcg agagcgaccc
     2041 cggcttcttc agcaacgcca tcgtggaggg cgccaagaag ttccccaaca ccgagttcgt
     2101 gaaggtgaag ggcctgcact tcctccagga ggacgccccc gacgagatgg gcaagtacat
     2161 caagagcttc gtggagagag tgctgaagaa cgagcagtaa gaattc
//
