LOCUS       sequence-80011-e        1821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Nano-lantern, a luminescent protein for 
            high-speed single-cell and whole body imaging, targeted to 
            mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern-mitochondria/pcDNA3
FEATURES             Location/Qualifiers
     source          1..1821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..81
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             103..174
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(196..198,199..201,202..714)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(241..262)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(502..521)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     849..870
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcatgt ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg
       61 ctcccagtgc cgcgcgccaa gatccattcg ttgggggatc tgtccgtcct gacgccgctg
      121 ctgctgcggg gcttgacagg ctcggcccgg cggctcccag tgccgcgcgc caagatccat
      181 tcgttggggg atccgatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      241 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      301 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
      361 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
      421 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      481 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      541 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      601 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
      661 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
      721 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      781 ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc caacgagaag
      841 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggggtaccaa ggtgtacgac
      901 cccgagcaga ggaagaggat gatcaccggc ccccagtggt gggccaggtg caagcagatg
      961 aacgtgctgg acagcttcat caactactac gacagcgaga agcacgccga gaacgccgtg
     1021 atcttcctgc acggcaacgc cactagcagc tacctgtgga ggcacgtggt gccccacatc
     1081 gagcccgtgg ccaggtgcat catccccgat ctgatcggca tgggcaagag cggcaagagc
     1141 ggcaacggca gctacaggct gctggaccac tacaagtacc tgaccgcctg gttcgagctt
     1201 ctgaacctgc ccaagaagat catcttcgtg ggccacgact ggggcgccgc cctggccttc
     1261 cactacgcct acgagcacca ggacaggatc aaggccatcg tgcacatgga gagcgtggtg
     1321 gacgtgatcg agagctggga cgagtggcca gacatcgagg aggacatcgc cctgatcaag
     1381 agcgaggagg gcgagaagat ggtgctggag aacaacttct tcgtggagac cgtgctgccc
     1441 agcaagatca tgagaaagct ggagcccgag gagttcgccg cctacctgga gcccttcaag
     1501 gagaagggcg aggtgagaag acccaccctg agctggccca gagagatccc cctggtgaag
     1561 ggcggcaagc ccgacgtggt gcagatcgtg agaaactaca acgcctacct gagagccagc
     1621 gacgacctgc ccaagctgtt catcgagggc gaccccggct tcttcagcaa cgccatcgtg
     1681 gagggcgcca agaagttccc caacaccgag ttcgtgaagg tgaagggcct gcacttcctc
     1741 caggaggacg cccccgacga gatgggcaag tacatcaaga gcttcgtgga gagagtgctg
     1801 aagaacgagc agtaactcga g
//
