LOCUS       sequence-80014-e        2176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=600nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_600/pRSETB
FEATURES             Location/Qualifiers
     source          1..2176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..526)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(53..74)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(314..333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1835..1912
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 ggatccgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
      481 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc cgggggtacc aaggtgtacg accccgagca
      721 gaggaagagg atgatcaccg gcccccagtg gtgggccagg tgcaagcaga tgaacgtgct
      781 ggacagcttc atcaactact acgacagcga gaagcacgcc gagaacgccg tgatcttcct
      841 gcacggcaac gccactagca gctacctgtg gaggcacgtg gtgccccaca tcgagcccgt
      901 ggccaggtgc atcatccccg atctgatcgg catgggcaag agcggcaaga gcggcaacgg
      961 cagctacagg ctgctggacc actacaagta cctgaccgcc tggttcgagc tcctgaacct
     1021 gcccaagaag atcatcttcg tgggccacga ctggggcgcc gccctggcct tccactacgc
     1081 ctacgagcac caggacagga tcaaggccat cgtgcacatg gagagcgtgg tggacgtgat
     1141 cgagagctgg gacgagtggc cagacatcga ggaggacatc gccctgatca agagcgagga
     1201 gggcgagaag atggtgctgg agaacaactt cttcgtggag accgtgctgc ccagcaagat
     1261 catgagaaag ctggagcccg aggagttcgc cgcctacctg gagcccttca aggagaaggg
     1321 cgaggtgaga agacccaccc tgagctggcc cagagagatc cccctggtga agggcccatg
     1381 gatgcatgac caactgacag aagagcagat tgcagagttc aaagaagcct tctcattatt
     1441 cgacaaggat ggggacggca ccatcaccac aaaggaactt ggcaccgtta tgaggtcgct
     1501 tggacaaaac ccaacggaag cagaattgca ggatatgatc aatgaagtcg atgctgatgg
     1561 caatggaacg atttactttc ctgaatttct tactatgatg gctagaaaaa tgaaggacac
     1621 agacagcgaa gaggaaatcc gagaagcatt ccgtgttttt gacaaggatg ggaacggcta
     1681 catcagcgct gctcagttac gtcacgtcat gacaaacctc ggggagaagt taacagatga
     1741 agaagttgat gaaatgataa gggaagcaga tatcgatggt gatggccaag taaactatga
     1801 agagtttgta caaatgatga cagcaaaggg ggggaagagg cgctggaaga aaaacttcat
     1861 tgccgtcagc gctgccaacc ggttcaagaa gatctccagc tccggggcac tggagctcgg
     1921 caagcccgac gtggtgcaga tcgtgagaaa ctacaacgcc tacctgagag ccagcgacga
     1981 cctgcccaag ctgttcatcg agggcgaccc cggcttcttc agcaacgcca tcgtggaggg
     2041 cgccaagaag ttccccaaca ccgagttcgt gaaggtgaag ggcctgcact tcctccagga
     2101 ggacgccccc gacgagatgg gcaagtacat caagagcttc gtggagagag tgctgaagaa
     2161 cgagcagtaa gaattc
//
